Protocolo para la identificación rápida y sensible de ricina en muestras ambientales ante una alerta... Sanid. mil. 2017; 73 (3) 157 un SDS-PAGE permitiendo descartar aquellas señales positivas debidas a la presencia de aglutinina o de cualquier otra proteína desconocida contenida en la muestra que pudiera reaccionar con los anticuerpos, porque no puede preverse todas las reacciones cruzadas que pudieran existir con muestras ambientales que pre-sentan una gran diversidad de matrices (agua, arena de distintas composiciones, suelos…). Así, este protocolo asegura la detección de ricina utilizando dos técnicas complementarias, ofreciendo, en un breve tiempo de respuesta del laboratorio, una buena sensibilidad en la de-tección. BIBLIOGRAFÍA 1. Audi J, Belson M; Patel M, Schier J, Osterloh J. Ricin Poisoning: a Compre-hensive Review. JAMA. 2005 Nov 9;294(18):2342-51. 2. He X, McMahon S, Henderson TD, Griffey SM, Cheng LW. Ricin toxicoki-netics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 2010 Sep 22;5(9):e12858. 3. Garber EA, Eppley RM, Stack ME, McLaughin A, Park DL. Feasibility of Immunodiagnostic Devices for the Detection of Ricin, Amanitin, and T-2 Toxin in Food. J Food Prot. 2005 Jun;68(6):1294-301. 4. Crompton R, Gall D. Georgi Malkov-Death in a pellet. Med Leg J. 1980;48(2):51-62. 5. Knight B. Ricin, a potent homicidal poison. Br Med J. 1979 Feb 3;1(6159):350-1. 6. Martin J. Chronology of Incidents Involving Ricin. CBR Weapons and WMD Terrorism News. 2008. Available from: http://cns.miis.edu/stories/ pdfs/080229_ricin.pdf. 7. Crowther JR. Walker JM editor. The ELISA Guidebook. Methods in Mo-lecular Biology. The International Atomic Energy Agency, Vienna, Austria. Humana Press Totowa, New Jersey. 2001;149. 8. Manns JM. SDS-Polyacrylamide Gel Electrophoresis (SDS-PA-GE) of Proteins. Current Protocols in Microbiology 2001. Availa-ble from: doi:10.1002/9780471729259.mca03ms22. http://dx.doi. org/10.1002/9780471729259.mca03ms22 9. Tanchou V. Review of methods for the rapid identification of pathogens in water samples ERNCIP thematic area Chemical & Biological Risks in the Water Sector Task 7, deliverable 1. 2014. Available from: http://publications. jrc.ec.europa.eu/repository/bitstream/JRC92395/lbna26881enn.pdf 10. Lian W, Wu D, Lim DV, Jin S. Sensitive detection of multiplex toxins using antibody microarray. Anal Biochem. 2010 Jun 15;401(2):271-9. 11. Shyu HF., Chiao DJ, Liu HW, Tang S S. Monoclonal Antibody-Based En-zyme Immunoassay for Detection of Ricin. Hybrid Hybridomics. 2002 Feb;21(1):69-73. 12. Simon S, Worbs S, Avondet MA , Tracz D M, Dano J, Schmidt L, Volland H, Dorner BG, Corbett CR. Recommended Immunological Assays to Screen for Ricin-Containing Samples. Toxins (Basel). 2015 Nov 26;7(12):4967-86. 13. Mechaly A, Cohen H, Cohen O, Mazor O. A biolayer interferometry-based assay for rapid and highly sensitive detection of biowarfare agents. Anal Bio-chem. 2016 Aug 1;506:22-7. 14. Anderson GP, Glaven RH, Algar WR, Susumu K, Stewart MH, Medintz IL, Goldman ER. Single domain antibody-quantum dot conjugates for ricin detection by both fluoroimmunoassay and surface plasmon resonance. Anal Chim Acta. 2013 Jul 5;786:132-8. 15. Worbs S, Skiba M, Bender J, Zeleny R, Schimmel H, Luginbühl W, Dorner B. G. An International Proficiency Test to Detect, Identify and Quantify Ricin in Complex Matrices. Toxins (Basel). 2015 Nov 26;7(12):4987-5010. 16. Cummings RD, Etzler ME. Types of Lectins. In: Essentials of Glycobiology. 2nd edition. 2009. Chapter 28. Available from http://www.ncbi.nlm.nih.gov/ books/NBK1940/
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