Investigación de Escherichia Coli productor de toxinas Shiga (STEC) en carnes y derivados cárnicos Sanid. mil. 2017; 73 (3) 151 cada vez más importante y los resultados obtenidos están en consonancia con esta afirmación27,28,29,54,55. La prevalencia de STEC obtenida en este estudio concuerda con la notificada a la EFSA por Italia y Austria45. Sin embargo, si tenemos en cuenta únicamente a los rumiantes salvajes, nues-tros resultados duplican lo notificado por estos países. Nuestra prevalencia es tres veces superior a la obtenida en los estudios de Piérard et al.56 y Miko et al.37, realizados en Bélgica y Alemania respectivamente. No hemos encontrado ningún estudio que investigase la pre-sencia de STEC en la carne de caza, cuya prevalencia superase a la de nuestro trabajo. Esto se debe a que la mayoría de estudios sobre prevalencia de STEC en especies cinegéticas se basan en el estudio de muestras de heces; la prevalencia de este microorganismo es mayor en el tracto intestinal y debe de producirse contaminación cruzada durante el faenado de la canal para que esta se contami-ne. Esto podría explicar que sólo hayamos encontrado prevalen-cias superiores a la nuestra en este tipo de trabajos28,57. Además, un factor limitante en la comparabilidad de nuestro estudio frente a otros proviene de las características del diseño de nuestra técnica diagnóstica; por un lado detectamos la presencia del gen Stx independientemente del tipo (1 ó 2), y por otro, inclui-mos el gen O157 como factor de virulencia junto con el resto (stx, eae). Si bien es cierto que en el caso de los estudios de prevalencia en cuanto a la presencia conjunta del gen O157 y el gen eae, nues-tros resultados concuerdan con lo publicado por Mora et al.58. BIBLIOGRAFÍA 1. EFSA BIOHAZ Panel, EFSA FIP & EFSA Scientific Committee. Scientific Opinion on the evaluation of the safety and efficacy of lactic acid for the removal of microbial surface contamination of beef carcasses, cuts and trim-mings. EFSA J. 9, 35 (2011). 2. EFSA. Urgent advice on the public health risk of Shiga-toxin producing Es-cherichia coli in fresh vegetables. EFSA J. 9, 50 (2011). 3. EFSA. Statement summarising the Conclusions and Recommendations from the Opinions on the safety of Irradiation of Food adopted by the BIOHAZ and CEF Panels. EFSA J. 9, 155 (2011). 4. Garrity, G. et al. Bergey’s Manual® of Systematic Bacteriology: Volume Two: The Proteobacteria. (Springer Science & Business Media, 2006). 5. Konowalchuk, J., Speirs, J. I. & Stavric, S. Vero response to a cytotoxin of Escherichia coli. Infect. Immun. 18, 775–779 (1977). 6. Levine, M. M. Escherichia coli that cause diarrhea: enterotoxigenic, entero-pathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J. Infect. Dis. 155, 377–389 (1987). 7. Bugarel, M., Beutin, L., Martin, A., Gill, A. & Fach, P. Micro-array for the identification of Shiga toxin-producing Escherichia coli (STEC) seropathoty-pes associated with Hemorrhagic Colitis and Hemolytic Uremic Syndrome in humans. Int. J. Food Microbiol. 142, 318–329 (2010). 8. Feng, P. C., Jinneman, K., Scheutz, F. & Monday, S. R. Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subty-pes. Appl. Environ. Microbiol. 77, 6699–6702 (2011). 9. Louie, M. et al. Sequence heterogeneity of the eae gene and detection of vero-toxin- producing Escherichia coli using serotype-specific primers. Epidemiol. Infect. 112, 449–461 (1994). 10. Garrido, P. et al. STEC-EPEC oligonucleotide microarray: a new tool for ty-ping genetic variants of the LEE pathogenicity island of human and animal Shiga toxin–producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains. Clin. Chem. 52, 192–201 (2006). 11. Garmendia, J., Frankel, G. & Crepin, V. F. Enteropathogenic and Enterohe-morrhagic Escherichia coli Infections: Translocation, Translocation, Translo-cation. Infect. Immun. 73, 2573–2585 (2005). 12. EFSA Working Group. Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic types. EFSA J 579, 1–61 (2007). 13. Mora, A. et al. HeLa-cell adherence patterns and actin aggregation of en-teropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles. Int. Microbiol. (2009). doi:10.2436/20.1501.01.104 14. Wu, G. et al. Genetic diversity among Escherichia coli O157: H7 isolates and identification of genes linked to human infections. Infect. Immun. 76, 845–856 (2008). 15. 15. Noris, M. & Remuzzi, G. Atypical Hemolytic–Uremic Syndrome. N. Engl. J. Med. 361, 1676–1687 (2009). 16. 16. Gyles, C. L. Shiga toxin-producing An overview. J. Anim. Sci. 85, E45–E62 (2007). 17. Kaspar, C., Doyle, M. E. & Archer, J. Food Safety Review: non-O157: H7 Shiga toxin-producing E. coli from meat and non-meat sources. Food Res. Inst. UW-Madison Dispon. En Httpfri Wisc EdudocspdfFRIBriefNonO157S-TEC410 Pdf Acceso 17-10-12 (2009). 18. Riley, L. W. et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308, 681–685 (1983). 19. Griffin, P. M. & Tauxe, R. V. The epidemiology of infections caused by Es-cherichia coli O157: H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol. Rev. 13, 60–98 (1991). 20. Alexandre, M., Prado, V., Ulloa, M. T., Arellano, C. & Rios, M. Detection of Enterohemorrhagic Escherichia coli in Meat Foods using DNA Probes, Enzyme-Linked Immunosorbent Assay and Polymerase Chain Reaction. J. Vet. Med. Ser. B 48, 321–330 (2001). 21. Armstrong, G. L., Hollingsworth, J. & Morris Jr, J. G. Emerging foodborne pathogens: Escherichia coli O157: H7 as a model of entry of a new patho-gen into the food supply of the developed world. Epidemiol. Rev. 18, 29–51 (1996). 22. Keene, W. E. et al. A prolonged outbreak of Escherichia coli O157: H7 in-fections caused by commercially distributed raw milk. J. Infect. Dis. 176, 815–818 (1997). 23. Upton, P. & Coia, J. Outbreak of Escherichia coli 0157 infection associated with pasteurised milk supply. The Lancet 344, 1015 (1994). 24. Mora, A. et al. Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157: H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). Int. J. Food Microbiol. 114, 204–210 (2007). 25. Doyle, M. P. & Schoeni, J. L. Isolation of Escherichia coli O157: H7 from retail fresh meats and poultry. Appl. Environ. Microbiol. 53, 2394– 2396 (1987). 26. Chapman, P. A. Sources of Escherichia coli O157 and experiences over the past 15 years in Sheffield, UK. J. Appl. Microbiol. 88, (2000). 27. Jay, M. T. et al. Escherichia coli O157: H7 in feral swine near spinach fields and cattle, central California coast. Emerg Infect Dis 13, 1908–1911 (2007). 28. Sánchez, S. et al. Detection and characterisation of Shiga toxin-producing Escherichia coli other than Escherichia coli O157: H7 in wild ruminants. Vet. J. 180, 384–388 (2009). 29. Rabatsky-Ehr, T. et al. Deer meat as the source for a sporadic case of Esche-richia coli O157: H7 infection. Emerg. Infect. Dis. 8, 525 (2002). 30. Hilborn, E. D. et al. A multistate outbreak of Escherichia coli O157: H7 in-fections associated with consumption of mesclun lettuce. Arch. Intern. Med. 159, 1758–1764 (1999). 31. Cody, S. H. et al. An outbreak of Escherichia coli O157: H7 infection from unpasteurized commercial apple juice. Ann. Intern. Med. 130, 202–209 (1999). 32. Michino, H. et al. Massive outbreak of Escherichia coli O157: H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts. Am. J. Epidemiol. 150, 787–796 (1999). 33. Kannali, M. A., Petric, M., Liin, C. & others. The association between idio-pathic hemolytic uremic syndrome and infection by verotoxin-producing Es-cherichia coll. J Infect Dis 151, 775–782 (1985). 34. Brooks, J. T. et al. Non-O157 Shiga toxin–producing Escherichia coli infec-tions in the United States, 1983–2002. J. Infect. Dis. 192, 1422–1429 (2005). 35. Martin, A. & Beutin, L. Characteristics of Shiga toxin-producing Escheri-chia coli from meat and milk products of different origins and association with food producing animals as main contamination sources. Int. J. Food Mi-crobiol. 146, 99–104 (2011).
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