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Diseño de oligonucleótidos sonda para la detección de virus de interés en biodefensa Sanid. mil. 2018 74 (3)  157 AGRADECIMIENTOS Laura González López agradece, al Instituto Nacional de Técnica Aeroespacial “Esteban Terradas”, la Beca de Formación concedida (Resolución 3D0/38136/2015, de 21 de septiembre). BIBLIOGRAFIA 1. Baños P. Terrorismo NRBQ. Visión Geopolítica analiza las posibilidades de un gran atentado nuclear, biológico, químico o radiológico, en Europa. In-ternet. Consultado 2016 Feb 22. Disponible en <http://www.tendencias21. net/Terrorismo-NRBQ_a42634.html>. 2. Thavaselvam D, Vijayaraghavan R. Biological warfare agents. J Pharm Bioallied Sci 2010;2(3):179-188. 3. Pérez Breña P. Gripe: estrategia para la prevención de un riesgo silente. Rev Esp Quimioter 2009; 22(1):5-6. 4. Clancy S. Genetics of the influenza virus. Nature Education 2008; 1(1):83. 5. Enders J. Cytopathology of virus infections: particular reference to tissue culture studies. Annu Rev Microbiol 1954;8:473-502 6. Zhang W, Evans D. Detection and identification of human influenza viruses by the polymerase chain reaction. J Virol Methods 1991;33:165-89 7. Holland J, Spindler K, Horodyski F, Grabau E, Nichol S, VandePol S. Rapid evolution of RNA genome. Science 1982; 215(4540):1577-85. 8. Smith DB, Inglis SC. The mutation rate and variability of eukaryotic viru-ses: an analytical review. J Gen Virol 1987;68: 2729-2740. 9. Mirski T, Bartoszcze M, Bielawska Drózd A, Gryko R, Kocik J, Niemcewicz M, et al. Microarrays – new possibilities for detecting biological factors ha-zardous for humans and animals, and for use in environmental protection. AAEM 2015;23(1):30-36. 10. Sultankulova KT, Kozhabergenov NS, Strochkov VM, Burashev YD, Sho-rayeva KA, Chervyakova OV, et al. New oligonucleotide microarray for ra-pid diagnosis of avian viral diseases. Virol J 2017;14(1). 11. McLoughlin KS. Microarrays for Pathogen Detection and Analysis. Brief Funct Genomics 2011; 10(6):342-53. 12. Wilson WD, Tanious FA, Fernandez-Saiz M, Rigl CT. Evaluation of drug-nucleic acid interactions by thermal melting curves. Methods Mol Biol 1997;90:219-240. 13. Zhang Z, Schwartz S, Wagner L, and Miller W. A greedy algorithm for alig-ning DNA sequences”, J Comput Biol 2000; 7(1-2):203-14. 14. Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Ostell J, Pruitt KD, et al. GenBank. Nucleic Acids Res 2018;46(D1): D41-D47. 15. Khanna M, Kumar P, Chugh L, Prasad A, Chhabra S. Evaluation of in-fluenza virus detection by direct enzyme immunoassay (EIA) and conventio-nal methods in asthmatic patients. J Commun Dis 2001;33:163-9 16. Bej A, Mahbubani M, Miller R, Dicesare J L, Haff L, Atlas R M. Multi-plex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water. Mol Cell Probes 1990;4:353–65. 17. Vandenvelde C, Verstraete M, Van Beers D. Fast multiplex polymerase chain reaction on boiled clinical samples for rapid viral diagnosis. J Virol Methods 1990;30:215–27. 18. Klotchenko SA, Vasin AV, Sandybaev NT, Plotnikova MA, Chervyakova OV, Smirnova EA, et al. Oligonucleotide microarray for subtyping of in-fluenza A viruses. J Phys Conf Ser 2012(1);345. 19. Quan P, Palacios G, Jabado OJ, Conlan S, Hirschberg DL, Pozo F, et al. Detection of respiratory viruses and subtype identification of influenza A viruses by greenechipresp oligonucleotide microarray. J Clin Microbiol 2007;45(8):2359-2364. 20. Erickson A, Fisher M, Furukawa-Stoffer T, Ambagala A, Hodko D, Pasick J, et al. A multiplex reverse transcription PCR and automated electronic microarray assay for detection and differentiation of seven viruses affecting swine. Transboundary Emer Dis 2018;65(2):272-283. 21. Wang D, Coscoy L, Zylberberg M, Avila PC, Boushey HA, Ganem D, De- Risi JL. Microarray-based detection and genotyping of viral pathogens. Proc Natl Acad Sci U S A 2002; 99(24):15687-92. 22. Sergeev N, Distler M, Courtney S, Al-Khaldi SF, Volokhov D, Chizhikov V, et al. Multipathogen oligonucleotide microarray for environmental and biodefense applications. Biosens Bioelectron 2004;20(4):684-698. 23. Uttamchandani M, Neo JL, Ong BNZ, Moochhala S. Applications of microarrays in pathogen detection and biodefence. Trends Biotechnol 2009;27(1):53-61.


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